COQ5 Recombinant antibody

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  WB analysis using 85890-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85890-1-RR (COQ5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

COQ5 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85890-1-RR

Clone No.250140E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial, EC:2.1.1.201, Ubiquinone biosynthesis methyltransferase COQ5

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85890-1-RR targets COQ5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COQ5 fusion protein Ag10203
• Full Name: coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
• Calculated Molecular Weight: 327 aa, 37 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC107874
• Gene Symbol: COQ5
• Gene ID (NCBI): 84274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5HYK3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COQ5 catalyzes the only C-methylation involved in the biosynthesis of coenzyme Q (Q or ubiquinone) in humans and yeast Saccharomyces cerevisiae. In humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases (PMID: 25152161). Endogenous human COQ5 protein primarily existed as a mature form (m-COQ5, ~32 kDa) without the mitochondrial targeting sequence (MTS) in the mitochondria of human cells, in addition to the minor precursor form (p-COQ5, 37 kDa) of the full-length protein (PMID: 27155576).