Multi-rAb® CORO1A Multi-Recombinant antibody

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• Multi-rAb® CORO1A Multi-Recombinant antibody (RMX00049)

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  WB analysis using RMX00049

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00049 (CORO1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using RMX00049

  IP result of anti-CORO1A (IP:RMX00049, 4ug; Detection:RMX00049 1:20000) with mouse brain tissue lysate 1960 ug.

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  IHC staining of mouse spleen using RMX00049

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using RMX00049 (CORO1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using RMX00049

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using RMX00049 (CORO1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using RMX00049

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CORO1A antibody (RMX00049) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CORO1A Multi-Recombinant Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. RMX00049

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

CLABP, Clipin A, CLIPINA, Clipin-A, CORO1

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00049 targets CORO1A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: coronin, actin binding protein, 1A
• Calculated Molecular Weight: 461 aa, 51 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC110374
• Gene Symbol: CORO1A
• Gene ID (NCBI): 11151
• RRID: AB_3750101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P31146
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).