COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G2B9

Cat no : 66351-1-Ig

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2



Tested Applications

Positive WB detected inRAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated HeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC013734
Gene symbol PTGS2
Gene ID (NCBI) 5743
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

Protocols

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Regen Ther

Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in vitro study using baicalein.

Authors - Chang-Chin Wu
humanWB,IHC

Oxid Med Cell Longev

Dihydroartemisinin Induces Ferroptosis in HCC by Promoting the Formation of PEBP1/15-LO.

Authors - Ying Su
human,mouseWB,IF

Cell Death Discov

Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis.

Authors - Jiazhi Yi
mouseWB

Oxid Med Cell Longev

Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2

Authors - Jie Jin
humanWB

Biomed Res Int

Emodin Alleviates Hydrogen Peroxide-Induced Inflammation and Oxidative Stress via Mitochondrial Dysfunction by Inhibiting the PI3K/mTOR/GSK3β Pathway in Neuroblastoma SH-SY5Y Cells.

Authors - Rui Li
ratWB

Pak J Pharm Sci

Platycodon grandiflorum extract attenuates lipopolysaccharide-induced acute lung injury via TLR4/NF-κBp65 pathway in rats.

Authors - Li Si-Cong