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COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody
COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3G2B9
Cat no : 66351-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated HeLa cells, HEK-293 cells |
Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse lung tissue, human breast cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 82 publications below |
IHC | See 11 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
Product Information
66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 |
Full Name | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
Calculated molecular weight | 604 aa, 68 kDa |
Observed molecular weight | 66-70 kDa |
GenBank accession number | BC013734 |
Gene symbol | PTGS2 |
Gene ID (NCBI) | 5743 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies. | ||
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | ||
Redox Biol The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3 | ||
J Nanobiotechnology Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Oxid Med Cell Longev Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2 |