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COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G2B9

Cat no : 66351-1-Ig

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2



Tested Applications

Positive WB detected inRAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated HeLa cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC013734
Gene symbol PTGS2
Gene ID (NCBI) 5743
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

Protocols

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.

Authors - David Pacheu-Grau
mouseWB

J Neuroinflammation

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Authors - Na Liu
ratWB

Chemosphere

Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity.

Authors - Zixuan Liu
mouseWB,IP

Emerg Microbes Infect

Gut dysbacteriosis attenuates resistance to Mycobacterium bovis infection by decreasing Cyclooxygenase 2 to inhibit endoplasmic reticulum stress.

Authors - Haoran Wang
mouse,humanWB,IF

EBioMedicine

Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

Authors - Zhongyang Lv
mouseWB

Int J Biol Macromol

Synergistic anti-inflammatory activity of apigenin and curcumin co-encapsulated in caseins assessed with lipopolysaccharide-stimulated RAW 264.7 macrophages.

Authors - Shan Hong