Carboxypeptidase A6 Polyclonal antibody

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  WB analysis of HepG2 using 13604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13604-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13604-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13604-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13604-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13604-1-AP

  Immunofluorescent analysis of HepG2 cells using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Carboxypeptidase A6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13604-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.17.-, CPA6, Carboxypeptidase B

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13604-1-AP targets Carboxypeptidase A6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4466

  Product name: Recombinant human CPA6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 130-437 aa of BC033684

  Sequence: SLSGYNYEVYHSLEEIQNWMHHLNKTHSGLIHMFSIGRSYEGRCLFILKLGRRSRLKRAVWIDCGIHAREWIGPAFCQWFVKEALLTYKSDPAMRKMLNHLYFYIMPVFNVDGYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCDEGASMHPCDDTYCGPFPESEPEVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVESAAYKAVNALQSVYGVRYRYGPASTTLYVSSGSSMDWAYKNGIPYAFAFELRDTGYFGFLLPEMLIKPTCTETMLAVKNITMHLLKKCP

• Full Name: carboxypeptidase A6
• Calculated Molecular Weight: 437 aa, 51 kDa
• Observed Molecular Weight: 65 kDa, 51 kDa
• GenBank Accession Number: BC033684
• Gene Symbol: CPA6
• Gene ID (NCBI): 57094
• RRID: AB_2261427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N4T0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carboxypeptidase A6 (CPA6) is an extracellular matrixbound metallocarboxypeptidase (CP) that has been implicated in Duane syndrome, a neurodevelopmental disorder in which the lateral rectus extraocularmuscle is not properly innervated.Consistent with a role inDuane syndrome, CPA6 is expressed in a number of chondrocytic and nervous tissues during embryogenesis.It has three isoforms.The product recognizes the C-terminal of this protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Arch Biochem Biophys; Carboxypeptidase A6 suppresses the proliferation and invasion of colorectal cancer cells and is negatively regulated by miR-96-3p; Authors - Xianren Wang