Tested Applications
| Positive WB detected in | mouse liver tissue, HepG2 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human colon cancer tissue, human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10672-1-AP targets Carboxypeptidase B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1110 Product name: Recombinant human CPB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-423 aa of BC007057 Sequence: YYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELSLVASEAVRAIEKTSKNTRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNV Predict reactive species |
| Full Name | carboxypeptidase B2 (plasma) |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC007057 |
| Gene Symbol | Carboxypeptidase B2 |
| Gene ID (NCBI) | 1361 |
| RRID | AB_2086000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96IY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Carboxypeptidase B2 antibody 10672-1-AP | Download protocol |
| WB protocol for Carboxypeptidase B2 antibody 10672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Carboxypeptidase B2 (CPB2/TAFI) polyclonal antibody (Cat# 10672-1-AP) has 4 total citations. These appear in International Journal of Biological Macromolecules, Marine Drugs, Neurochemical Research, and FEMS Immunology and Medical Microbiology. The research fields span alcohol-induced gastric mucosal injury, melanoma metastasis inhibition, chronic cerebral hypoperfusion and white matter damage, and the host fibrinolytic system. Applications cited include WB and IHC in mouse and rat models.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury | ||
Mar Drugs Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 Cells. | ||
Neurochem Res Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion | ||
FEMS Immunol Med Microbiol Secreted Bacillus anthracis proteases target the host fibrinolytic system. |
