Carboxypeptidase B2 Polyclonal antibody

Carboxypeptidase B2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10672-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Carboxypeptidase B2, carboxypeptidase B2 (plasma), Carboxypeptidase U, CPB2, CPU, PCPB, Plasma carboxypeptidase B, TAFI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10672-1-AP targets Carboxypeptidase B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1110

Product name: Recombinant human CPB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-423 aa of BC007057

Sequence: YYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELSLVASEAVRAIEKTSKNTRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNV

Predict reactive species
Full Name carboxypeptidase B2 (plasma)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC007057
Gene Symbol Carboxypeptidase B2
Gene ID (NCBI) 1361
RRIDAB_2086000
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase B2 antibody 10672-1-APDownload protocol
IHC protocol for Carboxypeptidase B2 antibody 10672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FEMS Immunol Med Microbiol

Secreted Bacillus anthracis proteases target the host fibrinolytic system.

Authors - Chung Myung-Chul MC
mouseWB

Neurochem Res

Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion

Authors - Yuhan Wang
ratIHC

Int J Biol Macromol

Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury

Authors - Fei Wang
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