CPE Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86111-1-RR (same clone as 86111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86111-1-RR (CPE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02472-1

  Sandwich ELISA standard curve of MP02472-1, Human CPE Recombinant Matched Antibody Pair - PBS only. 86111-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2989. 86111-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL

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  Sample test of MP02472-1

  Serum of fifteen individual healthy human donors was measured. The human CPE concentration of detected samples was determined to be 5.22 ng/mL with a range of 0.76 - 15.81 ng/mL.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 86111-1-RR (same clone as 86111-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CPE antibody (86111-1-RR, Clone: 250680F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

CPE Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86111-1-PBS

Clone No.250680F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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Product Information

86111-1-PBS targets CPE as part of a matched antibody pair:

MP02472-1: 86111-2-PBS capture and 86111-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CPE fusion protein Eg2989
• Full Name: carboxypeptidase E
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_001873.4
• Gene Symbol: Carboxypeptidase E
• Gene ID (NCBI): 1363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16870-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.