• Featured Product
  • KD/KO Validated

CPLX4 Polyclonal antibody

CPLX4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21222-1-AP

Synonyms

complexin 4, Complexin IV, CPLX4, CPX IV, CPXIV



Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inmouse eye tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21222-1-AP targets CPLX4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX4 fusion protein Ag15661
Full Name complexin 4
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC106887
Gene Symbol CPLX4
Gene ID (NCBI) 339302
RRIDAB_10733348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CPLX4 antibody 21222-1-APDownload protocol
IHC protocol for CPLX4 antibody 21222-1-APDownload protocol
IP protocol for CPLX4 antibody 21222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols