CPLX4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPLX4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21222-1-AP

Synonyms

complexin 4, Complexin IV, CPLX4, CPX IV, CPXIV

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Validation Data Gallery

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  WB analysis of Y79 using 21222-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 21222-1-AP

  WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.

• 

  WB analysis of Y79 using 21222-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 21222-1-AP

  IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.

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  IHC staining of mouse eye using 21222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 21222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21222-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: mouse eye tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21222-1-AP targets CPLX4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPLX4 fusion protein Ag15661
• Full Name: complexin 4
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 21-23 kDa
• GenBank Accession Number: BC106887
• Gene Symbol: CPLX4
• Gene ID (NCBI): 339302
• RRID: AB_10733348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.