Tested Applications
| Positive WB detected in | HEK-293 cells, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16440-1-AP targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9761 Product name: Recombinant human CPM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 99-426 aa of BC022276 Sequence: YLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHSAAT Predict reactive species |
| Full Name | carboxypeptidase M |
| Calculated Molecular Weight | 443 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa, 62-70 kDa |
| GenBank Accession Number | BC022276 |
| Gene Symbol | Carboxypeptidase M |
| Gene ID (NCBI) | 1368 |
| RRID | AB_2084046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14384 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Carboxypeptidase M antibody 16440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Carboxypeptidase M Polyclonal antibody (Cat# 16440-1-AP) has 1 total citation. It appears in Science Advances (2025), in a study investigating how FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance L-asparagine production. The research field is oncology, specifically breast cancer metastasis and tumor microenvironment biology.
| Species | Application | Title |
|---|---|---|
Sci Adv FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production |
