Carboxypeptidase M Polyclonal antibody

• 

  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16440-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Carboxypeptidase M Polyclonal Antibody for WB, ELISA

Cat No. 16440-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CPM, CarboxypeptidaseM, EC:3.4.17.12

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16440-1-AP targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9761

  Product name: Recombinant human CPM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 99-426 aa of BC022276

  Sequence: YLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHSAAT

• Full Name: carboxypeptidase M
• Calculated Molecular Weight: 443 aa, 51 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC022276
• Gene Symbol: Carboxypeptidase M
• Gene ID (NCBI): 1368
• RRID: AB_2084046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14384
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen