CPNE1 Polyclonal antibody

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  WB analysis using 10126-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10126-2-AP (CPNE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10126-2-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10126-2-AP (CPNE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CPNE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10126-2-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Chromobindin 17, Copine 1, Copine I, Copine-1, copineI

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, mouse stomach tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

10126-2-AP targets CPNE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0139

  Product name: Recombinant human CPNE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 25-289 aa of BC001142

  Sequence: KSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVG

• Full Name: copine I
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC001142
• Gene Symbol: CPNE1
• Gene ID (NCBI): 8904
• RRID: AB_2084202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99829
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; Copine-III interacts with ErbB2 and promotes tumor cell migration.; Authors - C Heinrich
human	WB,IHC	Carcinogenesis; Copine 1 predicts poor clinical outcomes by promoting M2 macrophage activation in ovarian cancer; Authors - Bo Sheng
human	WB	MedComm (2020); Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1.; Authors - Yang Wang
human	WB	Front Biosci (Landmark Ed); Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer; Authors - Yelin Song ; KO Validated
human	WB	Cell Death Discov; Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p; Authors - Jianye Zhong