CPNE3 Recombinant monoclonal antibody, PBS Only

Name: CPNE3 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83820-5-PBS-100UG

CPNE3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 83820-5-PBS

Clone No.240871E5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

240871E5, Copine 3, Copine III, Copine-3, copineIII

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIPIF/ICCFC (Intra)Affinity

WB analysis using 83820-5-RR (same clone as 83820-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83820-5-RR (CPNE3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
WB analysis of A431 using 83820-5-RR (same clone as 83820-5-PBS)
WB result of CPNE3 antibody (83820-5-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPNE3 transfected A431 cells. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)
IP result of anti-CPNE3 (IP:83820-5-RR, 4ug; Detection:83820-5-RR 1:2000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
IF Staining of A431 using 83820-5-RR (same clone as 83820-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPNE3 antibody (83820-5-RR, Clone: 240871E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83820-5-RR
Biolayer interferometry (BLl) kinetic assays of 83820-5-RR against Human CPNE3 were performed. The affinity constant is below 1 pM.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83820-5-PBS targets CPNE3 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1634 Product name: Recombinant human CPNE3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-537 aa of BC036242 Sequence: EASRSSPVEFECINEKKRQKKKSYKNSGVISVKQCEITVECTFLDYIMGGCQLNFTVGVDFTGSNGDPRSPDSLHYISPNGVNEYLTALWSVGLVIQDYDADKMFPAFGFGAQIPPQWQVSHEFPMNFNPSNPYCNGIQGIVEAYRSCLPQIKLYGQTNFSPIINHVARFAAAATQQQTASRYFVLLIITDGVITDLDETRQAIVNASRLPMSIIIVGVGGADFSAMEFLDGDGGSLHSPLGEVAIRDIVQFVPFRQFQNAPKEALAQCVLAEIPQQVVGYFNTYKLLPPKNPATKQQKQ Predict reactive species
Full Name	copine III
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC036242
Gene Symbol	CPNE3
Gene ID (NCBI)	8895
RRID	AB_3671405
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	O75131
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. Copine III (CPNE3) has also been identified as a ligand of ERBB2 and plays an important role in various carcinogenesis. The high expression of CPNE3 is an adverse prognostic biomarker for acute myeloid leukemia (PMID: 28670859).