CPNE4 Polyclonal antibody

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  WB analysis using 16075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16075-1-AP (CPNE4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 16075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPNE4 antibody (16075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CPNE4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16075-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Copine 4, Copine 8, copine IV, COPN4, CPN4, CPNE4

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16075-1-AP targets CPNE4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9037

  Product name: Recombinant human CPNE4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 217-557 aa of BC014396

  Sequence: LCSGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKNYKNSGTVILNLCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQPNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQGVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDMADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPFRNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEMYESSRTLAP

• Full Name: copine IV
• Calculated Molecular Weight: 557 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC014396
• Gene Symbol: CPNE4
• Gene ID (NCBI): 131034
• RRID: AB_3085480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96A23
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. CPNE4 (Copine 4) belongs to the copine family. This antibody is specific to CPNE4.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cajsa (Verified Customer) (09-17-2024)

Great antibody. Image of enteric neuronal nuclei (green, Baf53 reporter) and Cpna4 (red).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Myenteric plexus of adult mouse intestine

CPNE4 Antibody Immunofluorescence validation (1:300 dilution) in Myenteric plexus of adult mouse intestine (Cat no:16075-1-AP)