Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16075-1-AP targets CPNE4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9037 Product name: Recombinant human CPNE4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-557 aa of BC014396 Sequence: LCSGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKNYKNSGTVILNLCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQPNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQGVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDMADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPFRNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEMYESSRTLAP Predict reactive species |
| Full Name | copine IV |
| Calculated Molecular Weight | 557 aa, 62 kDa |
| Observed Molecular Weight | 62-65 kDa |
| GenBank Accession Number | BC014396 |
| Gene Symbol | CPNE4 |
| Gene ID (NCBI) | 131034 |
| RRID | AB_3085480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A23 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. CPNE4 (Copine 4) belongs to the copine family. This antibody is specific to CPNE4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPNE4 antibody 16075-1-AP | Download protocol |
| WB protocol for CPNE4 antibody 16075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for 16075-1-AP is a 5-star rating from an immunofluorescence application. The reviewer used a 1:300 dilution on myenteric plexus of adult mouse intestine, imaging enteric neuronal nuclei (green, Baf53 reporter) alongside Cpna4 (red). They described it as a "great antibody," indicating strong performance and clear staining in their IF experiment. Overall, the feedback is positive, highlighting reliable results in neuronal tissue imaging.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cajsa (Verified Customer) (09-17-2024) | Great antibody. Image of enteric neuronal nuclei (green, Baf53 reporter) and Cpna4 (red).
 |
