Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13782-1-AP targets CPNE6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4789 Product name: Recombinant human CPNE6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 258-557 aa of BC018046 Sequence: QEMQWDCINPKYRDKKKNYKSSGTVVLAQCTVEKVHTFLDYIMGGCQISFTVAIDFTASNGDPRSSQSLHCLSPRQPNHYLQALRAVGGICQDYDSDKRFPAFGFGARIPPNFEVSHDFAINFDPENPECEEISGVIASYRRCLPQIQLYGPTNVAPIINRVAEPAQREQSTGQATKYSVLLVLTDGVVSDMAETRTAIVRASRLPMSIIIVGVGNADFSDMRLLDGDDGPLRCPRGVPAARDIVQFVPFRDFKDAAPSALAKCVLAEVPRQVVEYYASQGISPGAPRPCTLATTPSPSP Predict reactive species |
| Full Name | copine VI (neuronal) |
| Calculated Molecular Weight | 557 aa, 62 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC018046 |
| Gene Symbol | CPNE6 |
| Gene ID (NCBI) | 9362 |
| RRID | AB_2292172 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95741 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CPNE6 antibody 13782-1-AP | Download protocol |
| WB protocol for CPNE6 antibody 13782-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPNE6 (Copine-6) Polyclonal antibody (catalog number 13782-1-AP) has 5 total citations. These appear in Phytomedicine, EMBO Journal, Cell Reports, Frontiers in Molecular Neuroscience, and Journal of Neurological Sciences. The associated research fields span neuroscience, including hippocampal neural plasticity and cognitive function, sensory neuron heat sensitivity, AMPA receptor exocytosis, and epilepsy.
| Species | Application | Title |
|---|---|---|
Phytomedicine 20(S)-protopanaxadiol targets brain-enriched adenylate kinase 5 to improve cognitive function via regulating hippocampal neural plasticity. | ||
EMBO J Copine-6 is a TRPM3 escort protein controlling the sensitivity of sensory neurons to noxious heat | ||
Front Mol Neurosci Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice | ||
J Neurol Sci Increased expression of copine VI in patients with refractory epilepsy and a rat model. |
