CPNE7 Polyclonal antibody

• 

  WB analysis using 17396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17396-1-AP (CPNE7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17396-1-AP

  mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 17396-1-AP (CPNE7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

CPNE7 Polyclonal Antibody for WB, ELISA

Cat No. 17396-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Copine 7, copine VII, CPNE7

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

17396-1-AP targets CPNE7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11313

  Product name: Recombinant human CPNE7 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 212-558 aa of BC035334

  Sequence: VSLSSLCSCEETRPLKCLVWDYDSRGKHDFIGEFSTTFEEMQKAFEEGQAQWDCVNPKYKQKRRSYKNSGVVVLADLKFHRVYSFLDYIMGGCQIHFTVAIDFTASNGDPRNSCSLHYINPYQPNEYLKALVSVGEICQDYDSDKRFSALGFGARIPPKYEVSHDFAINFNPEDDECEGIQGVVEAYQNCLPRVQLYGPTNVAPIISKVARVAAAEESTGKASQYYILLILTDGVVTDMADTREAIVRASRLPMSIIIVGVGNADFTDMQVLDGDDGVLRSPRGEPALRDIVQFVPFRELKNASPAALAKCVLAEVPKQVVEYYSHRGLPPRSLGVPAGEASPGCTP

• Full Name: copine VII
• Calculated Molecular Weight: 558 aa, 62 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC035334
• Gene Symbol: CPNE7
• Gene ID (NCBI): 27132
• RRID: AB_2878403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBL6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
human	WB	Front Oncol; Copine 7 promotes colorectal cancer proliferation through PKM2 interaction and MAPK signaling pathway; Authors - Tianwen Yu ; KD Validated
human	WB	Cell Death Dis; CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription; Authors - Liangbo Zhao ; KD Validated
human	WB	J Neurochem; CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation; Authors - Jie Yang