Tested Applications
| Positive WB detected in | COLO 320 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17396-1-AP targets CPNE7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11313 Product name: Recombinant human CPNE7 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 212-558 aa of BC035334 Sequence: VSLSSLCSCEETRPLKCLVWDYDSRGKHDFIGEFSTTFEEMQKAFEEGQAQWDCVNPKYKQKRRSYKNSGVVVLADLKFHRVYSFLDYIMGGCQIHFTVAIDFTASNGDPRNSCSLHYINPYQPNEYLKALVSVGEICQDYDSDKRFSALGFGARIPPKYEVSHDFAINFNPEDDECEGIQGVVEAYQNCLPRVQLYGPTNVAPIISKVARVAAAEESTGKASQYYILLILTDGVVTDMADTREAIVRASRLPMSIIIVGVGNADFTDMQVLDGDDGVLRSPRGEPALRDIVQFVPFRELKNASPAALAKCVLAEVPKQVVEYYSHRGLPPRSLGVPAGEASPGCTP Predict reactive species |
| Full Name | copine VII |
| Calculated Molecular Weight | 558 aa, 62 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC035334 |
| Gene Symbol | CPNE7 |
| Gene ID (NCBI) | 27132 |
| RRID | AB_2878403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPNE7 antibody 17396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPNE7 Polyclonal antibody (Cat# 17396-1-AP) has 4 citations. These appear in Cell Death & Disease, Journal of Neurochemistry, Molecular Oncology, and Frontiers in Oncology. The research fields span colorectal cancer tumorigenesis and metastasis, cancer cell proliferation via MAPK signaling, and amyloidogenesis related to Alzheimer's disease. All studies used the antibody for Western blot in human samples, with two employing RNAi validation.
| Species | Application | Title |
|---|---|---|
Cell Death Dis CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription
| ||
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Front Oncol Copine 7 promotes colorectal cancer proliferation through PKM2 interaction and MAPK signaling pathway
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