Tested Applications
| Positive WB detected in | mouse liver tissue, HeLa cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67630-1-Ig targets CPS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | carbamoyl-phosphate synthetase 1, mitochondrial |
| Calculated Molecular Weight | 165 kDa |
| Observed Molecular Weight | 140-165 kDa |
| GenBank Accession Number | BC140943 |
| Gene Symbol | CPS1 |
| Gene ID (NCBI) | 1373 |
| RRID | AB_2882831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPS1 antibody 67630-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPS1 Monoclonal antibody (2A12A4), catalog number 67630-1-Ig, has 2 total citations. These appear in Journal of Ethnopharmacology and Journal of Animal Science. The associated research fields include metabolic dysfunction-associated fatty liver disease (targeting the DNMT-urea cycle axis in mice) and animal nutrition (dietary starch replacement with soybean oil in weaned Rex rabbits). Both citations used the antibody for Western blot applications.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Mechanism of Jianpi Qinghua granule in ameliorating metabolic dysfunction-associated fatty liver disease by targeting the DNMT-urea cycle axis. | ||
J Anim Sci Partially replacing dietary starch with soybean oil improved production performance of weaned Rex rabbits |
