CPSF2 Recombinant monoclonal antibody

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  WB analysis using 86513-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86513-3-RR (CPSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86513-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPSF2 antibody (86513-3-RR, Clone: 251387H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86513-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86513-3-RR against Human CPSF2 were performed. The affinity constant is 0.487 nM.

CPSF2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86513-3-RR

Clone No.251387H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CPSF 100 kDa subunit, CPSF100, KIAA1367

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, Ramos cells, RAW 264.7 cells, C6 cells, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86513-3-RR targets CPSF2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11950

  Product name: Recombinant human CPSF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 433-782 aa of BC070095

  Sequence: SAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPELQATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQLIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYRIRDLLYEQYAIV

• Full Name: cleavage and polyadenylation specific factor 2, 100kDa
• Calculated Molecular Weight: 782 aa, 88 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC070095
• Gene Symbol: CPSF2
• Gene ID (NCBI): 53981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2I0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673)