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CPSF3 Polyclonal antibody, PBS Only (11609-1-PBS)

Filter: AllWBIPIHC

WB analysis using 11609-1-AP (same clone as 11609-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 11609-1-AP (same clone as 11609-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 11609-1-AP (same clone as 11609-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 11609-1-AP (same clone as 11609-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 11609-1-AP (same clone as 11609-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 11609-1-AP (same clone as 11609-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 11609-1-AP (same clone as 11609-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CPSF3 Polyclonal antibody, PBS Only

Name: CPSF3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11609-1-PBS

CPSF3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 11609-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11609-1-PBS targets CPSF3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2173 Product name: Recombinant human CPSF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 337-684 aa of BC020211 Sequence: SRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH Predict reactive species
Full Name	cleavage and polyadenylation specific factor 3, 73kDa
Calculated Molecular Weight	684 aa, 77 kDa
Observed Molecular Weight	77 kDa
GenBank Accession Number	BC020211
Gene Symbol	CPSF3
Gene ID (NCBI)	51692
RRID	AB_2292188
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UKF6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).