Tested Applications
| Positive WB detected in | mouse colon tissue, mouse spleen tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15860-1-AP targets INTS11 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8365 Product name: Recombinant human INT11 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 250-600 aa of BC007978 Sequence: ILLETFWERMNLKVPIYFSTGLTEKANHYYKLFIPWTNQKIRKTFVQRNMFEFKHIKAFDRAFADNPGPMVVFATPGMLHAGQSLQIFRKWAGNEKNMVIMPGYCVQGTVGHKILSGQRKLEMEGRQVLEVKMQVEYMSFSAHADAKGIMQLVGQAEPESVLLVHGEAKKMEFLKQKIEQELRVNCYMPANGETVTLPTSPSIPVGISLGLLKREMAQGLLPEAKKPRLLHGTLIMKDSNFRLVSSEQALKELGLAEHQLRFTCRVHLHDTRKEQETALRVYSHLKSVLKDHCVQHLPDGSVTVESVLLQAAAPSEDPGTKVLLVSWTYQDEELGSFLTSLLKKGLPQAPS Predict reactive species |
| Full Name | cleavage and polyadenylation specific factor 3-like |
| Calculated Molecular Weight | 600 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC007978 |
| Gene Symbol | INT11 |
| Gene ID (NCBI) | 54973 |
| RRID | AB_2084537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TA45 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrator complex subunit 11 (INT11) is a protein that in humans is encoded by the INTS11 gene. INTS11, also known as Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L), contains a metallo-β-lactamase and β-CASP domain that, together, coordinate two zinc ions within the active site to promote its RNA endonuclease activity. INTS11 is the catalytic subunit of the Integrator (INT) complex, which is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. INT11 has 5 isoforms with the molecular mass of 56 and 65-68 kDa (PMID: 34516911, PMID: 37054711).
Publications
What published studies show
The INTS11 Polyclonal antibody (catalog no. 15860-1-AP) has 6 total citations. These appear in high-impact journals including Nature, Science Bulletin, Nature Communications, Molecular Cell, Journal of Virology, and Open Medicine. The associated research fields span transcription regulation, the Integrator endonuclease complex, RNA polymerase II elongation, pluripotency, heterochromatin, viral (KSHV) replication, somatic hypermutation, and gastric cancer prognosis/tumor microenvironment. Applications cited include Western blot and ChIP.
| Species | Application | Title |
|---|---|---|
Sci Bull (Beijing) GABPA recruits the Integrator endonuclease complex to promote transcription elongation. | ||
Nat Commun Transcriptional competence defines the heterochromatin nucleating potential of isolated MSR units | ||
Mol Cell Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during development.
| ||
J Virol Essential function of the integrator complex in Kaposi's sarcoma-associated herpesvirus lytic replication.
| ||
Open Med (Wars) Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer
| ||
Reviews
What customers say
The single review for this antibody is highly positive, with a 5-star rating. The user performed a Western Blot on HuH7 cells at a 1:1000 dilution and reported that the antibody works well and is specific to its target, producing a nice clear band. Overall, the feedback highlights strong specificity and reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Britt (Verified Customer) (05-03-2023) | Antibody works well and is specific to target. Got a nice clear band.
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