INT11 Polyclonal antibody

• Featured Product
• KD/KO Validated

INT11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 15860-1-AP

Synonyms

RC 68, INTS11, EC:3.1.27.-, CPSF3-like protein, CPSF3L

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Validation Data Gallery

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  WB analysis of mouse colon using 15860-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INT11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15860-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INT11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15860-1-AP

  Immunofluorescent analysis of HepG2 cells using 15860-1-AP (INT11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse spleen tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

15860-1-AP targets INT11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INT11 fusion protein Ag8365
• Full Name: cleavage and polyadenylation specific factor 3-like
• Calculated Molecular Weight: 600 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC007978
• Gene Symbol: INT11
• Gene ID (NCBI): 54973
• RRID: AB_2084537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 11 (INT11) is a protein that in humans is encoded by the INTS11 gene. INTS11, also known as Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L), contains a metallo-β-lactamase and β-CASP domain that, together, coordinate two zinc ions within the active site to promote its RNA endonuclease activity. INTS11 is the catalytic subunit of the Integrator (INT) complex, which is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. INT11 has 5 isoforms with the molecular mass of 56 and 65-68 kDa (PMID: 34516911, PMID: 37054711).

Publications

Species	Application	Title
human	WB	Open Med (Wars); Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer; Authors - Xiaoxia Tong ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Britt (Verified Customer) (05-03-2023)

Antibody works well and is specific to target. Got a nice clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HuH7