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  • KD/KO Validated

CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10225-1-AP

Synonyms

CRABP II, CRABP2, RBP6



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human skin cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10225-1-AP targets CRABP2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309
Full Name cellular retinoic acid binding protein 2
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2085455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
WB protocol for CRABP2 antibody 10225-1-APDownload protocol
IHC protocol for CRABP2 antibody 10225-1-APDownload protocol
IF protocol for CRABP2 antibody 10225-1-APDownload protocol
IP protocol for CRABP2 antibody 10225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

A single progenitor population switches behavior to maintain and repair esophageal epithelium.

Authors - David P Doupé
humanWB

Mol Cell

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Authors - Ai Kaiho-Soma
humanWB,IF

Neuron

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

Authors - Alana I Mendelsohn
mouseIF

Nat Commun

The developing mouse coronal suture at single-cell resolution.

Authors - D'Juan T Farmer
mouseIF

Dev Cell

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

Authors - John DeSisto
humanIF

Stem Cell Reports

BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.

Authors - Kai Kretzschmar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse embryonic section
FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse brain tissue