CRAT Polyclonal antibody

• Featured Product
• KD/KO Validated

CRAT Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15170-1-AP

Synonyms

EC:2.3.1.137, CAT1, CAT, Carnitine O-acetyltransferase, Carnitine acetylase

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Validation Data Gallery

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  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15170-1-AP

  IP result of anti-CRAT (IP:15170-1-AP, 4ug; Detection:15170-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of mouse liver using 15170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15170-1-AP (CRAT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15170-1-AP (CRAT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15170-1-AP (CRAT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15170-1-AP (CRAT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle, rat heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse brain tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:800-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below

Product Information

15170-1-AP targets CRAT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRAT fusion protein Ag7422
• Full Name: carnitine acetyltransferase
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC000723
• Gene Symbol: CRAT
• Gene ID (NCBI): 1384
• RRID: AB_2229978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRAT, also named as CAT1, belongs to the carnitine/choline acetyltransferase family. It is specific for short chain fatty acids. CRAT seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CRAT is an active forms for carnitine acetyltransferase. This antibody can bind the close sequences genes.

Publications

Species	Application	Title
rat	WB	Cell Metab; Dissociation of Muscle Insulin Resistance from Alterations in Mitochondrial Substrate Preference.; Authors - Joongyu D Song
		Cancer Res; Organelle-Derived Acetyl-CoA Promotes Prostate Cancer Cell Survival, Migration, and Metastasis via Activation of Calmodulin Kinase II.; Authors - Guoyu Yu
goat	WB	Redox Biol; TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis.; Authors - Xutong Sun ; KD Validated
sheep	WB	Antioxid Redox Signal; Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow.; Authors - Sun Xutong X
mouse	WB	Mol Metab; Carnitine acetyltransferase (CRAT) expression in macrophages is dispensable for nutrient stress sensing and inflammation.; Authors - Emily L Goldberg
mouse	WB	Heliyon; Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction; Authors - Yasuro Furuichi