Filter: AllIHCIF-PIF/ICC

IHC staining of mouse eye using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 26598-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 26598-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB1 antibody (26598-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 26598-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CRB1 Polyclonal antibody

Name: CRB1 Polyclonal antibody
Brand: Proteintech
SKU: 26598-1-AP
Rating: 5.00 (1 reviews)

CRB1 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Cat No. 26598-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in	mouse eye tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26598-1-AP targets CRB1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24512 Product name: Recombinant human CRB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-188 aa of BC136271 Sequence: NNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPVCICPAGYAGRFCEIDHDECASSPCQNGAVCQDGIDGYSCFCVPGYQGRHCDLEVD Predict reactive species
Full Name	crumbs homolog 1 (Drosophila)
Calculated Molecular Weight	1406 aa, 154 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC136271
Gene Symbol	CRB1
Gene ID (NCBI)	23418
RRID	AB_2918104
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P82279
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).