CREB3L1,OASIS Polyclonal antibody

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CREB3L1,OASIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11235-2-AP Copied!

Publications (20)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CREB3L1, OASIS, cAMP-responsive element-binding protein 3-like protein 1, Cyclic AMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance

• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 11235-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 11235-2-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 11235-2-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 11235-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11235-2-AP targets CREB3L1,OASIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1757

  Product name: Recombinant human CREB3L1,OASIS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 170-519 aa of BC015781

  Sequence: PHQAPGEMTQLPVIKAEPLEVNQFLKVTPEDLVQMPPTPPSSHGSDSDGSQSPRSLPPSSPVRPMARSSTAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTKLPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQLQKLQTLVTNKISRPYKMAATQTGTCLMVAALCFVLVLGSLVPCLPEFSSGSQTVKEDPLAADGVYTASQMPSRSLLFYDDGAGLWEDGRSTLLPMEPPDGWEINPGGPAEQRPRDHLQHDHLDSTHETTKYLSEAWPKDGGNGTSPDFSHSKEWFHDRDLGPNTTIKLS

• Full Name: cAMP responsive element binding protein 3-like 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 55-58 kDa and 84 kDa
• GenBank Accession Number: BC015781
• Gene Symbol: CREB3L1/OASIS
• Gene ID (NCBI): 90993
• RRID: AB_2086042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96BA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Publications

What published studies show

The anti-CREB3L1 antibody (Cat# 11235-2-AP) has 20 citations across journals including J Immunother Cancer, J Adv Res, Int J Oral Sci, Sci Signal, Breast Cancer Res, Commun Biol, BMC Cancer, Endocrinology, Epigenetics, PLoS One, and others. Research fields cover oncology (breast, bladder, glioma, gastric, and thyroid cancers), endoplasmic reticulum stress, epigenetics, extracellular matrix biology, fibrosis, odontoblastic differentiation, mitochondrial homeostasis, and cognitive function.

Automatically generated from the 20 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Int J Oral Sci; CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B; Authors - Yuanyuan Li
mouse	WB	J Adv Res; The m(6)A modification mediates the imbalance of mitochondrial homeostasis and apoptosis induced by Benzo[b]fluoranthene in mouse spermatocytes: mitophagy versus mitochondrial damage.; Authors - Chao-Feng Shi
human	WB	J Immunother Cancer; POSTN+ cancer-associated fibroblasts determine the efficacy of immunotherapy in hepatocellular carcinoma; Authors - Hao Wang
		Int J Biol Macromol; A prospective study on the inhibition of gastric cancer by 164.88° recombinant humanized type III collagen through remodeling of extracellular matrix.; Authors - Yixuan Song
mouse	WB	Sci Signal; High glucose impairs cognitive function through Creb3 O-GlcNAcylation and increased lactate production.; Authors - Jingxi Xu
human	WB,IHC	Breast Cancer Res; Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers.; Authors - Alison K Ward