CRELD1 Polyclonal antibody

CRELD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29903-1-AP

Synonyms

AVSD2, CIRRIN, CRELD1, DKFZp566D213

---

Validation Data Gallery

• 

  WB analysis of rat lung using 29903-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 29903-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 29903-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 29903-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 29903-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse lung tissue, rat lung tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29903-1-AP targets CRELD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRELD1 fusion protein Ag31593
• Full Name: cysteine-rich with EGF-like domains 1
• Calculated Molecular Weight: 45KD
• Observed Molecular Weight: 45 kDa, 70-75 kDa
• GenBank Accession Number: NM_001031717
• Gene Symbol: CRELD1
• Gene ID (NCBI): 78987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).