CRELD1 Polyclonal antibody, PBS Only

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• CRELD1 Polyclonal antibody, PBS Only (29903-1-PBS)

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  WB analysis of HepG2 using 29903-1-AP (same clone as 29903-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 29903-1-AP (same clone as 29903-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 29903-1-AP (same clone as 29903-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 29903-1-AP (same clone as 29903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29903-1-AP (same clone as 29903-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

CRELD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29903-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

29903-1-PBS targets CRELD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31593

  Product name: Recombinant human CRELD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 218-362 aa of NM_001031717

  Sequence: CARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE

• Full Name: cysteine-rich with EGF-like domains 1
• Calculated Molecular Weight: 45KD
• Observed Molecular Weight: 45 kDa, 70-75 kDa
• GenBank Accession Number: NM_001031717
• Gene Symbol: CRELD1
• Gene ID (NCBI): 78987
• RRID: AB_2923619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HD1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).