CoraLite® Plus 488-conjugated CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10944

Synonyms

Corticoliberin, Corticotropin releasing factor, CRF, CRH

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-10944

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-10944

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10944 targets CRH/CRF in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRH/CRF fusion protein Ag1371
• Full Name: CRH/CRF
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC011031
• Gene Symbol: CRH
• Gene ID (NCBI): 1392
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.