CDK12/CRKRS Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK12/CRKRS Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 26816-1-AP

Synonyms

CDC2 related protein kinase 7, CDK12, CDK12/CRKRS, CRK7, CRKR, CRKRS, KIAA0904

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Validation Data Gallery

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  WB analysis of HeLa using 26816-1-AP

  WB result of CDK12/CRKRS antibody (26816-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK12/CRKRS transfected HeLa cells.

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  WB analysis using 26816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26816-1-AP (CDK12/CRKRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26816-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26816-1-AP (CRKRS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26816-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26816-1-AP (CRKRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, Jurkat cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

The immunogen of 26816-1-AP is CDK12/CRKRS Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK12/CRKRS fusion protein Ag25323
• Full Name: Cdc2-related kinase, arginine/serine-rich
• Calculated Molecular Weight: 1490 aa, 164 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC150265
• Gene Symbol: CRKRS
• Gene ID (NCBI): 51755
• RRID: AB_2880645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).

Publications

Species	Application	Title
human	WB	Eur J Cancer; CDK12 inhibition enhances sensitivity of HER2+ breast cancers to HER2-tyrosine kinase inhibitor via suppressing PI3K/AKT.; Authors - Hui Li
human	WB	iScience; THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate; Authors - Mareike Polenkowski
human		Int J Med Sci; Expression pattern of CDK12 protein in gastric cancer and its positive correlation with CD8+ cell density and CCL12 expression.; Authors - Jun Ji
human	WB	Cell Death Discov; JWA suppresses proliferation in trastuzumab-resistant breast cancer by downregulating CDK12.; Authors - Yan Liang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

M (Verified Customer) (06-29-2021)

No Signal at all at the right size. A couple of non specific bands showed up at lower molecular weights.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver lysate

CDK12/CRKRS Antibody Western Blot validation (1:1000 dilution) in Human Liver lysate (Cat no:26816-1-AP)