CRLF3 Polyclonal antibody

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  WB analysis using 11567-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11567-1-AP (CRLF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CRLF3 Polyclonal Antibody for WB, ELISA

Cat No. 11567-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CREME 9, CREME9, CRLF3, CYTOR4, FRWS, P48

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Tested Applications

• Positive WB detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11567-1-AP targets CRLF3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2144

  Product name: Recombinant human CRLF3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 141-442 aa of BC023567

  Sequence: DSLPEVPLLVDVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWCKVDDDFTAQDYRLQFRKCTSNHFEDVYVGSETEFIVLHIDPNVDYQFRVCARGDGRQEWSPWSVPQIGHSTLVPHEWTAGFEGYSLSSRRNIALRNDSESSGVLYSRAPTYFCGQTLTFRVETVGQPDRRDSIGVCAEKQDGYDSLQRDQAVCISTNGAVFVNGKEMTNQLPAVTSGSTVTFDIEAVTLGTTSNNEGGHFKLRVTISSNNREVVFDWLLDQSCGSLYFGCSFFYPGWKVLVF

• Full Name: cytokine receptor-like factor 3
• Calculated Molecular Weight: 442 aa, 50 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC023567
• Gene Symbol: CRLF3
• Gene ID (NCBI): 51379
• RRID: AB_3085377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IUI8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokine receptor-like factor 3 (CRLF3), a member of the group 1 in the prototypic class I cytokine receptors, contains the conserved cytokine receptor motif WSXWS, a single transmembrane segment and an intracellular Janus kinase (JAK) docking site (PMID: 31680856). CRLF3 is expressed in most normal human tissues and many tumor cell lines and has been reported to negatively regulate cell cycle progression (PMID: 19427400). CRLF3 has been identified as a human neuroprotective EV-3 (Epo) receptor (PMID: 37168680). It is involved neurogenesis, neuron survival, and dendritic development (PMID: 34233200).