CRLS1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

CRLS1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (2)

Cat no : 14845-1-AP

Synonyms

C20orf155, Cardiolipin synthase, cardiolipin synthase 1, CLS, CLS1, CRLS1, CRLS1-Specific, GCD10, Protein GCD10 homolog

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Validation Data Gallery

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  WB analysis using 14845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14845-1-AP (CRLS1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14845-1-AP (CRLS1-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human heart tissue, human liver cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below

Product Information

14845-1-AP targets CRLS1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cardiolipin synthase 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_019095
• Gene Symbol: CRLS1
• Gene ID (NCBI): 54675
• RRID: AB_2085336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiolipin (CL) is a mitochondrial-specific double negatively charged phospholipid which is intricately involved in regulating bioenergetic efficiency. In eukaryotes, CL is synthesized by cardiolipin synthase (CRLS1 or CLS1) which catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form CL and glycerol. Recently it has been reported that transgenic expression of CRLS1 accelerates cardiolipin remodeling, improves mitochondrial function, modulates mitochondrial signaling, and attenuates mitochondrial dysfunction during diabetes, thereby identifying CRLS1 as a novel therapeutic target to attenuate mitochondrial dysfunction in diabetic myocardium. The predicted molecular weight of CRLS1 is around 32-35 kDa, while a 50 kDa protein with CRLS1 activity had been observed in liver isolation, which may represent an isoform of CRLS1 (20652826).

Publications

Species	Application	Title
human	WB	EMBO J; Cristae formation is a mechanical buckling event controlled by the inner mitochondrial membrane lipidome; Authors - Kailash Venkatraman ; KD Validated
mouse	WB	J Nutr Biochem; Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus.; Authors - Lei Wu
mouse	WB	Front Mol Neurosci; Cardiolipin externalization mediates prion protein (PrP) peptide 106-126-associated mitophagy and mitochondrial dysfunction; Authors - Dongming Yang
human	WB	J Biol Chem; Myocardial regulation of lipidomic flux by cardiolipin synthase: setting the beat for bioenergetic efficiency.; Authors - Kiebish Michael A MA
mouse	WB	Neural Regen Res; Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms.; Authors - Mushfiquddin Khan
rat	WB	Physiol Behav; Exercise ameliorates high-fat diet-induced insulin resistance accompanied by changes in protein levels of hepatic ATF3-related signaling in rats.; Authors - Zhiwei Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

siiting (Verified Customer) (03-20-2022)

very good , clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

FH

siiting (Verified Customer) (03-18-2022)

very good , clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue