|Positive WB detected in||Neuro-2a cells, HeLa cells, HEK-293T cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
27647-1-AP targets CRTC1 in WB,ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRTC1 fusion protein Ag26494|
|Full Name||CREB regulated transcription coactivator 1|
|Calculated molecular weight||67 kDa|
|Observed molecular weight||68-75 kDa|
|GenBank accession number||BC017075|
|Gene ID (NCBI)||23373|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.