CRTC1 Polyclonal antibody

CRTC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27647-1-AP

Synonyms

TORC1/CRTC1, TORC-1, TORC1, TORC 1, MECT1

---

Validation Data Gallery

• 

  WB analysis of Neuro-2a using 27647-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27647-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 27647-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 27647-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27647-1-AP (CRTC1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Neuro-2a cells, HeLa cells, HEK-293T cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27647-1-AP targets CRTC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRTC1 fusion protein Ag26494
• Full Name: CREB regulated transcription coactivator 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC017075
• Gene Symbol: CRTC1
• Gene ID (NCBI): 23373
• RRID: AB_2880938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.

Publications

Species	Application	Title
human	WB	Autophagy; Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing.; Authors - Lei Qiang
rat	WB	Brain Res; The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats; Authors - Wanyu Tu