CRTC1 Polyclonal antibody

CRTC1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 27647-1-AP

Synonyms

CRTC1, KIAA0616, MECT1, TORC 1, TORC1, Transducer of CREB protein 1, WAMTP1



Tested Applications

Positive WB detected inNeuro-2a cells, HeLa cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27647-1-AP targets CRTC1 in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTC1 fusion protein Ag26494
Full Name CREB regulated transcription coactivator 1
Calculated molecular weight 67 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC017075
Gene symbol CRTC1
Gene ID (NCBI) 23373
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.

Protocols

Product Specific Protocols
WB protocol for CRTC1 antibody 27647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing.

Authors - Lei Qiang