CRX Polyclonal antibody

CRX Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 12047-1-AP

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, diencephalon/mesencephalon homeobox 1, DMBX1, LCA7, OTX3



Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12047-1-AP targets CRX in WB, IP, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRX fusion protein Ag2677
Full Name cone-rod homeobox
Calculated molecular weight 299 aa, 32 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC016664
Gene symbol CRX
Gene ID (NCBI) 1406
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

Protocols

Product Specific Protocols
WB protocol for CRX antibody 12047-1-APDownload protocol
IP protocol for CRX antibody 12047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, CoIP

J Neurosci

The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina.

Authors - Tomohiro Masuda