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CRYBB1 Monoclonal antibody, PBS Only

CRYBB1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F12B12

Cat no : 60273-1-PBS

Synonyms

Beta B1 crystallin, Beta crystallin B1, CATCN3, CRYBB1, crystallin, beta B1

Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using CRYBB1 Monoclonal antibody (60273-1-Ig)

    WB analysis of C6 using 60273-1-Ig (same clone as 60273-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using CRYBB1 Monoclonal antibody (60273-1-Ig)

    WB analysis of mouse eye using 60273-1-Ig (same clone as 60273-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60273-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CRYBB1 Monoclonal antibody (60273-1-Ig)

    IF Staining of hTERT-RPE1 using 60273-1-Ig (same clone as 60273-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60273-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60273-1-PBS targets CRYBB1 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRYBB1 fusion protein Ag4722
Full Name crystallin, beta B1
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC036790
Gene Symbol CRYBB1
Gene ID (NCBI) 1414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.