Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32233-1-AP targets CRYGD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37473 Product name: Recombinant human CRYGD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-120 aa of NM_006891.3 Sequence: MGKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNE Predict reactive species |
| Full Name | crystallin, gamma D |
| Calculated Molecular Weight | 21kDa，174aa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | NM_006891.3 |
| Gene Symbol | CRYGD |
| Gene ID (NCBI) | 1421 |
| RRID | AB_3742550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P07320 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CRYGD (Crystallin Gamma D) is a structural protein predominantly expressed in the lens of the eye. It plays a critical role in maintaining lens transparency and refractive index. Mutations in the CRYGD gene are associated with inherited forms of congenital cataracts, making it a genetic target for studying lens development and cataract pathogenesis. It is not a conventional therapeutic drug target but serves as a key marker in ocular disease research.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYGD antibody 32233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
