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CSE1L Polyclonal antibody, PBS Only (22219-1-PBS)

Filter: AllWBIPIHC

WB analysis using 22219-1-AP (same clone as 22219-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis using 22219-1-AP (same clone as 22219-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis using 22219-1-AP (same clone as 22219-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 22219-1-AP (same clone as 22219-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis of RAW264.7 using 22219-1-AP (same clone as 22219-1-PBS)
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 22219-1-AP (same clone as 22219-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)
IP result of anti-CSE1L (IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CSE1L Polyclonal antibody, PBS Only

Name: CSE1L Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 22219-1-PBS

CSE1L Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 22219-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

22219-1-PBS targets CSE1L in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17556 Product name: Recombinant human CSE1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 627-971 aa of BC109313 Sequence: RITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL Predict reactive species
Full Name	CSE1 chromosome segregation 1-like (yeast)
Calculated Molecular Weight	971 aa, 110 kDa
Observed Molecular Weight	100-110 kDa
GenBank Accession Number	BC109313
Gene Symbol	CSE1L
Gene ID (NCBI)	1434
RRID	AB_10950892
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55060
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.