CSNK1G1 Polyclonal antibody, PBS Only

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• CSNK1G1 Polyclonal antibody, PBS Only (16384-1-PBS)

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  WB analysis using 16384-1-AP (same clone as 16384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 16384-1-AP (same clone as 16384-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

CSNK1G1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16384-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

16384-1-PBS targets CSNK1G1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9395

  Product name: Recombinant human CSNK1G1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-398 aa of BC017236

  Sequence: VGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQRHK

• Full Name: casein kinase 1, gamma 1
• Calculated Molecular Weight: 422 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC017236
• Gene Symbol: CSNK1G1
• Gene ID (NCBI): 53944
• RRID: AB_2087300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCP0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.