CSNK2A1 Monoclonal antibody, PBS Only

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  WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68200-1-Ig (same clone as 68200-1-PBS)

  WB result of CSNK2A1 antibody (68200-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 68200-1-Ig (same clone as 68200-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68200-1-PBS

Clone No.1D5E8

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1D5E8, CK II alpha, CK2a, CK2A1, CK2α

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Product Information

68200-1-PBS targets CSNK2A1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29062

  Product name: Recombinant human CSNK2A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-391 aa of BC011668

  Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ

• Full Name: casein kinase 2, alpha 1 polypeptide
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC011668
• Gene Symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P68400
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.