Tested Applications
| Positive WB detected in | LNCaP cells, A549 cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68200-1-Ig targets CSNK2A1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rabbit samples.
| Tested Reactivity | human, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29062 Product name: Recombinant human CSNK2A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-391 aa of BC011668 Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ Predict reactive species |
| Full Name | casein kinase 2, alpha 1 polypeptide |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC011668 |
| Gene Symbol | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| RRID | AB_2935289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P68400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
| WB protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CSNK2A1 Monoclonal antibody (1D5E8), catalog number 68200-1-Ig, has 3 total citations. These appear in Advanced Science, Veterinary Microbiology, and F1000Research. The associated research fields include myocardial fibrosis and mitochondrial dysfunction, viral egress mechanisms, and antibody validation for western blot, immunoprecipitation, and immunofluorescence applications.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) CK2α Deficiency Drives Myocardial Fibrosis via Desmin-Induced Mitochondrial Dysfunction. | ||
Vet Microbiol Pseudorabies virus EP0 recruits TECPR2 and CK2 to promote COPII accumulation and viral egress. | ||
F1000Res A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence
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