CSRP3 Polyclonal antibody
CSRP3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10721-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, human heart tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
10721-1-AP targets CSRP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CSRP3 fusion protein Ag1136 |
Full Name | cysteine and glycine-rich protein 3 (cardiac LIM protein) |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC005900 |
Gene Symbol | CSRP3 |
Gene ID (NCBI) | 8048 |
RRID | AB_2292475 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for CSRP3 antibody 10721-1-AP | Download protocol |
IHC protocol for CSRP3 antibody 10721-1-AP | Download protocol |
IP protocol for CSRP3 antibody 10721-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
FASEB J Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts. | ||
Hum Mol Genet Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. | ||
J Gene Med CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family. | ||
Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH WEI (Verified Customer) (03-03-2022) | Very strong single band by comparing the KO tissues
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