CSRP3 Polyclonal antibody

CSRP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10721-1-AP

Synonyms

Cardiac LIM protein, CLP, CMD1M, CMH12, CRP3, CSRP3, Cysteine rich protein 3, LIM domain protein, cardiac, LMO4, MLP, Muscle LIM protein



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10721-1-AP targets CSRP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSRP3 fusion protein Ag1136
Full Name cysteine and glycine-rich protein 3 (cardiac LIM protein)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC005900
Gene Symbol CSRP3
Gene ID (NCBI) 8048
RRIDAB_2292475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSRP3 antibody 10721-1-APDownload protocol
IHC protocol for CSRP3 antibody 10721-1-APDownload protocol
IP protocol for CSRP3 antibody 10721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.

Authors - Julia Straubinger
mouseWB

Hum Mol Genet

Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.

Authors - Aymeric Ravel-Chapuis
humanIHC

J Gene Med

CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family.

Authors - Hao Huang
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

WEI (Verified Customer) (03-03-2022)

Very strong single band by comparing the KO tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse Heart