CSRP3 Polyclonal antibody

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  WB analysis of mouse heart using 10721-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10721-1-AP

  IP result of anti-CSRP3 (IP:10721-1-AP, 4ug; Detection:10721-1-AP 1:2000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10721-1-AP (CSRP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10721-1-AP (CSRP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10721-1-AP (CSRP3 antibody) at dilution of 1:100 (under 10x lens).

CSRP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10721-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Cardiac LIM protein, CMD1M, CRP3, Cysteine and glycine-rich protein 3, Cysteine rich protein 3

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10721-1-AP targets CSRP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1136

  Product name: Recombinant human CSRP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-194 aa of BC005900

  Sequence: MPNWGGGAKCGACEKTVYHAEEIQCNGRSFHKTCFHCMACRKALDSTTVAAHESEIYCKVCYGRRYGPKGIGYGQGAGCLSTDTGEHLGLQFQQSPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE

• Full Name: cysteine and glycine-rich protein 3 (cardiac LIM protein)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005900
• Gene Symbol: CSRP3
• Gene ID (NCBI): 8048
• RRID: AB_2292475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50461
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. The cytoplasmic CSRP3 serves as a scaffold protein that primarily interacts and co-localizes with different Z-disc components of myofibrils (PMID: 31979369, 17519359).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	FASEB J; Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.; Authors - Julia Straubinger
mouse	WB	Hum Mol Genet; Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.; Authors - Aymeric Ravel-Chapuis
human	IHC	J Gene Med; CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family.; Authors - Hao Huang
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-03-2022)

Very strong single band by comparing the KO tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse Heart