Cystatin A Recombinant antibody

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  WB analysis of U2OS using 84953-4-RR

  U2OS cells were subjected to SDS PAGE followed by western blot with 84953-4-RR (CSTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84953-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84953-4-RR against Human Cystatin A were performed. The affinity constant is 0.286 nM.

Cystatin A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84953-4-RR

Clone No.242558F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CSTA, 242558F10, Stefin-A, Cystatin-A, N-terminally processed, Cystatin-A

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Tested Applications

• Positive WB detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84953-4-RR targets Cystatin A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cystatin A fusion protein Ag8733
• Full Name: cystatin A (stefin A)
• Calculated Molecular Weight: 98 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC010379
• Gene Symbol: Cystatin A
• Gene ID (NCBI): 1475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01040
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes.