Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10823-1-AP

Synonyms

CPI B, CST6, CSTB, Cystatin B, cystatin B (stefin B), EPM1, PME, Stefin B, STFB



Tested Applications

Positive WB detected inhuman saliva tissue, HepG2 cells, human liver tissue, human lymph tissue, human placenta tissue, human spleen tissue, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10823-1-AP targets Cystatin B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag1262
Full Name cystatin B (stefin B)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003370
Gene symbol CSTB
Gene ID (NCBI) 1476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 10823-1-APDownload protocol
IHC protocol for Cystatin B antibody 10823-1-APDownload protocol
IF protocol for Cystatin B antibody 10823-1-APDownload protocol
IP protocol for Cystatin B antibody 10823-1-APDownload protocol
FC protocol for Cystatin B antibody 10823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols