CSTF3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSTF3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24290-1-AP

Synonyms

CF 1 77 kDa subunit, CSTF 77, CSTF 77 kDa subunit, CSTF3

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Validation Data Gallery

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  WB analysis using 24290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24290-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 24290-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 24290-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 24290-1-AP

  IP result of anti-CSTF3 (IP:24290-1-AP, 4ug; Detection:24290-1-AP 1:500) with A431 cells lysate 1200ug.

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, HEK-293 cells
• Positive IP detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

24290-1-AP targets CSTF3 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSTF3 fusion protein Ag17930
• Full Name: cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
• Calculated Molecular Weight: 717 aa, 83 kDa
• Observed Molecular Weight: 77-83 kDa
• GenBank Accession Number: BC059948
• Gene Symbol: CSTF3
• Gene ID (NCBI): 1479
• RRID: AB_2879475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min ; KD Validated
human	WB	Comput Struct Biotechnol J; Alternative polyadenylation regulates the translation of metabolic and inflammation-related proteins in adipose tissue of gestational diabetes mellitus; Authors - Bingnan Chen
human	IF	Cell Death Dis; CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1; Authors - Xin Luo