NY-ESO-1 Polyclonal antibody

NY-ESO-1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19521-1-AP

Synonyms

CTAG1A, LAGE2, ESO1, CTAG1, CTAG

---

Validation Data Gallery

• 

  WB analysis of human testis using 19521-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19521-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 19521-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 19521-1-AP

  IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IF Staining of HepG2 using 19521-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTAG1A antibody 19521-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: human testis tissue, HT-1080 cells, NCI-H1299 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

19521-1-AP targets NY-ESO-1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cancer/testis antigen 1A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 16-25 kDa
• GenBank Accession Number: NM_139250
• Gene Symbol: NY-ESO-1
• Gene ID (NCBI): 246100
• RRID: AB_10638144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.

Publications

Species	Application	Title
human	WB	Cancer Immunol Res; CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance; Authors - Liyan Lao
human	WB	Nat Cancer; The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer.; Authors - Jiang Liu
human	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
human	IF,WB	Cell Death Dis; STING agonist diABZI enhances the cytotoxicity of T cell towards cancer cells; Authors - Ling Wang