NY-ESO-1 Polyclonal antibody

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  WB analysis of human testis using 19521-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 19521-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19521-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 19521-1-AP

  IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 19521-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19521-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NY-ESO-1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 19521-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CTAG1A, Autoimmunogenic cancer/testis antigen NY-ESO-1, CTAG, CTAG1, ESO1

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Tested Applications

• Positive WB detected in: human testis tissue, HT-1080 cells, NCI-H1299 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

19521-1-AP targets NY-ESO-1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cancer/testis antigen 1A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 16-25 kDa
• GenBank Accession Number: NM_139250
• Gene Symbol: NY-ESO-1
• Gene ID (NCBI): 246100
• RRID: AB_10638144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78358
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
human	WB	Cancer Immunol Res; CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance; Authors - Liyan Lao
human	WB	Nat Cancer; The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer.; Authors - Jiang Liu
human	WB,IF	Cell Death Dis; STING agonist diABZI enhances the cytotoxicity of T cell towards cancer cells; Authors - Ling Wang