CTAGE5 Polyclonal antibody

CTAGE5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55279-1-AP

Synonyms

cTAGE family member 5, CTAGE family, member 5, CTAGE5, MEA11, MEA6, MGEA, MGEA11, MGEA6, MIA2, Protein cTAGE 5

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Validation Data Gallery

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  WB analysis using 55279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 55279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human brain tissue, PC-3 cells, human testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55279-1-AP targets CTAGE5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: CTAGE family, member 5
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_005930
• Gene Symbol: CTAGE5
• Gene ID (NCBI): 4253
• RRID: AB_11183044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.