CTBS Polyclonal antibody

CTBS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12599-1-AP

Synonyms

EC:3.2.1.-, Di-N-acetylchitobiase, CTB

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Validation Data Gallery

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  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of SMMC-7721 using 12599-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12599-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12599-1-AP (CTBS Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 12599-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTBS antibody 12599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells, mouse colon tissue
• Positive IHC detected in: human prostate cancer tissue, human liver cancer tissue, human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12599-1-AP targets CTBS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTBS fusion protein Ag3228
• Full Name: chitobiase, di-N-acetyl-
• Calculated Molecular Weight: 12 kDa, 43 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC024007
• Gene Symbol: CTBS
• Gene ID (NCBI): 1486
• RRID: AB_10638783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.