CTDSP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CTDSP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10952-1-AP

Synonyms

NLIIF, NLI IF, NIF3, EC:3.1.3.16, CTDSP 1

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Validation Data Gallery

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  WB analysis using 10952-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10952-1-AP (CTDSP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

10952-1-AP targets CTDSP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTDSP1 fusion protein Ag1410
• Full Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-33 kDa
• GenBank Accession Number: BC012977
• Gene Symbol: CTDSP1
• Gene ID (NCBI): 58190
• RRID: AB_2087073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTDSP1 also named as NIF3, NLIIF, or SCP1, is a 261 amino acid protein, which contains one FCP1 homology domain. CTDSP1 localizes in the nucleus and colocalizes with RNA polymerase II. CTDSP1 is expressed in non-neuronal tissues. It is highly expressed in skeletal muscle, spleen, lung and placenta. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2.

Publications

Species	Application	Title
human	WB	J Biol Chem; Phosphatase activity of Small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).; Authors - Nathaniel Tate Burkholder ; KD Validated
human	WB	Cell Death Dis; MicroRNA-26a/b have protective roles in oral lichen planus.; Authors - Jie Du
human	WB	Iran J Basic Med Sci; MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma; Authors - Chunming Zhao
mouse	WB	Cell Mol Neurobiol; Elabela, a Novel Peptide, Exerts Neuroprotective Effects Against Ischemic Stroke Through the APJ/miR-124-3p/CTDSP1/AKT Pathway; Authors - Kang-Long Zhang