CoraLite® Plus 488-conjugated CTNNA3 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated CTNNA3 Recombinant monoclonal antibody (CL488-87022)

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  IF Staining of HepG2 using CL488-87022

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CTNNA3 antibody (CL488-87022, Clone: 252141F2 ) at dilution of 1:200.

CTNNA3 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-87022

Clone No.252141F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF/ICC

Synonyms

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-87022 targets CTNNA3 in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5008

  Product name: Recombinant human CTNNA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-376 aa of BC030646

  Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL

• Full Name: catenin (cadherin-associated protein), alpha 3
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030646
• Gene Symbol: CTNNA3
• Gene ID (NCBI): 29119
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UI47
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).