CTRB1 Polyclonal antibody

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• CTRB1 Polyclonal antibody (24939-1-AP)

CTRB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24939-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

chymotrypsinogen B1, CTRB, CTRB1

• 

  WB analysis of mouse pancreas using 24939-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat pancreas using 24939-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat pancreas using 24939-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 24939-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24939-1-AP targets CTRB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8604

  Product name: Recombinant human CTRB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-263 aa of BC005385

  Sequence: SGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAAN

• Full Name: chymotrypsinogen B1
• Calculated Molecular Weight: 263 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005385
• Gene Symbol: CTRB1
• Gene ID (NCBI): 1504
• RRID: AB_2879809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17538
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CTRB1 Polyclonal antibody (Cat# 24939-1-AP) has 1 total citation. It appears in Biol Open (2021), where it was used for WB and IF in mouse samples. The cited study investigates exocrine pancreas proteases and their role in regulating β-cell proliferation in zebrafish ciliopathy models and murine systems, placing the research in the fields of pancreatic biology and ciliopathy.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB，IF	Biol Open; Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.; Authors - Timothy L Hostelley