CTRB1 Polyclonal antibody

CTRB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24939-1-AP

Synonyms

chymotrypsinogen B1, CTRB, CTRB1

---

Validation Data Gallery

• 

  WB analysis of mouse pancreas using 24939-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 24939-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 24939-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 24939-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 24939-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 24939-1-AP is CTRB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTRB1 fusion protein Ag8604
• Full Name: chymotrypsinogen B1
• Calculated Molecular Weight: 263 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005385
• Gene Symbol: CTRB1
• Gene ID (NCBI): 1504
• RRID: AB_2879809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB，IF	Biol Open; Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.; Authors - Timothy L Hostelley