Tested Applications
| Positive WB detected in | mouse pancreas tissue, BxPC-3 cells |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14856-1-AP targets CTRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6644 Product name: Recombinant human CTRL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC063475 Sequence: MLLLSLTLSLVLLGSSWGCGIPAIKPALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHCNVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLKLASPAQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSCQGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN Predict reactive species |
| Full Name | chymotrypsin-like |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC063475 |
| Gene Symbol | CTRL |
| Gene ID (NCBI) | 1506 |
| RRID | AB_10640303 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CTRL antibody 14856-1-AP | Download protocol |
| IHC protocol for CTRL antibody 14856-1-AP | Download protocol |
| WB protocol for CTRL antibody 14856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CTRL (Chymotrypsin-like protease) polyclonal antibody (Cat# 14856-1-AP) has 3 citations. These appear in Thromb Haemost, Biol Open, and Oncotarget. The associated research fields include hematology and cardiac surgery (plasma proteolytic cascade during neonatal cardiopulmonary bypass), pancreatic biology and developmental ciliopathy models (exocrine pancreas proteases regulating β-cell proliferation), and oncology (serine protease levels as indicators of colorectal cancer progression).
| Species | Application | Title |
|---|---|---|
Thromb Haemost Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery. | ||
Biol Open Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems. | ||
Oncotarget The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression. |
