CTRL Polyclonal antibody

CTRL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14856-1-AP

Synonyms

EC:3.4.21.-, CTRL1, Chymotrypsin-like protease CTRL-1, chymotrypsin like

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Validation Data Gallery

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  WB analysis of mouse pancreas using 14856-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14856-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14856-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 14856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 14856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of BxPC-3 using 14856-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14856-1-AP (CTRL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, BxPC-3 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14856-1-AP targets CTRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTRL fusion protein Ag6644
• Full Name: chymotrypsin-like
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC063475
• Gene Symbol: CTRL
• Gene ID (NCBI): 1506
• RRID: AB_10640303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

Publications

Species	Application	Title
human	WB	Thromb Haemost; Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery.; Authors - Susan A Maroney
mouse	WB	Biol Open; Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.; Authors - Timothy L Hostelley
		Oncotarget; The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression.; Authors - Yingying Xie