Cathepsin F Polyclonal antibody

• Featured Product
• KD/KO Validated

Cathepsin F Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11055-1-AP

Synonyms

Cathepsin F, CATSF, CTSF

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Validation Data Gallery

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  IHC staining of human prostate cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11055-1-AP (Cathepsin F antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue, human heart tissue, human stomach cancer tissue, mouse heart tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

11055-1-AP targets Cathepsin F in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin F fusion protein Ag1521
• Full Name: cathepsin F
• Calculated Molecular Weight: 53 kDa and 34 kDa
• GenBank Accession Number: BC011682
• Gene Symbol: Cathepsin F
• Gene ID (NCBI): 8722
• RRID: AB_2292434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSF(Cathepsin F) is a member of the papain family of cysteine proteases. These enzymes represent a major component of the lysosomal proteolytic system. They are synthesized as inactive precursors consisting of a signal sequence, a propeptide, and a catalytically active mature region. Cathepsins are routed to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The deduced 484-amino acid cathepsin F protein has a signal sequence and potential glycosylation sites.

Publications

Species	Application	Title
rat	IHC	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao ; KD Validated
human	IHC	Brain Res Bull; Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.; Authors - Saghizadeh Mehrnoosh M
human	IHC	Proc Natl Acad Sci U S A; A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB; Authors - Yuqi Lin
human	WB	Oncol Res; Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells; Authors - Ce Ji ; KD Validated