CUL1 Recombinant monoclonal antibody

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  WB analysis of A549 using 82474-1-RR

  WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells.

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  WB analysis using 82474-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82474-1-RR

  IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug.

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  Affinity and Kinetic Characterization of 82474-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

• Featured Product
• KD/KO Validated

CUL1 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 82474-1-RR

Clone No.5K17

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IP, ELISA

Synonyms

Cullin-1, Cullin1, CUL-1, 5K17

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:17000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82474-1-RR targets CUL1 in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3585

  Product name: Recombinant human CUL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 111-462 aa of BC034318

  Sequence: YCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

• Full Name: cullin 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC034318
• Gene Symbol: CUL1
• Gene ID (NCBI): 8454
• RRID: AB_3086481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13616
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.