Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, MCF-7 cells, PC-3 cells, Raji cells, SW 1990 cells, TF-1 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67175-1-Ig targets CUL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28654 Product name: Recombinant human CUL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 341-745 aa of BC009591 Sequence: ESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA Predict reactive species |
| Full Name | cullin 2 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC009591 |
| Gene Symbol | CUL2 |
| Gene ID (NCBI) | 8453 |
| RRID | AB_2882471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13617 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| IHC protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| WB protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CUL2 Monoclonal antibody (1A6G9), catalog number 67175-1-Ig, has a total of 2 citations. These citations appear in Pharmacological Research and FEBS Journal. The associated research fields include cancer therapy (targeting NEDD8 conjugating enzyme UBC12) and cullin neddylation complex assembly involving TRAF2, reflecting applications in proteasome pathway biology and drug discovery.
| Species | Application | Title |
|---|---|---|
