CUL2 Monoclonal antibody

CUL2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-Ig

Synonyms

CUL 2, CUL2, cullin 2



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67175-1-Ig targets CUL2 in WB, IHC,ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL2 fusion protein Ag28654
Full Name cullin 2
Calculated molecular weight 87 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC009591
Gene symbol CUL2
Gene ID (NCBI) 8453
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1–3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL2 antibody 67175-1-IgDownload protocol
IHC protocol for CUL2 antibody 67175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols