CUL2 Monoclonal antibody, PBS Only

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  WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

  WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis using 67175-1-Ig (same clone as 67175-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67175-1-Ig (same clone as 67175-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 67175-1-Ig (same clone as 67175-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67175-1-Ig (same clone as 67175-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67175-1-Ig (same clone as 67175-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67175-1-PBS

Clone No.1A6G9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CUL 2, CUL-2, cullin 2, Cullin-2

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Product Information

67175-1-PBS targets CUL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28654

  Product name: Recombinant human CUL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 341-745 aa of BC009591

  Sequence: ESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA

• Full Name: cullin 2
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC009591
• Gene Symbol: CUL2
• Gene ID (NCBI): 8453
• RRID: AB_2882471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13617
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.